Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human Total VAV1 Detection Kit, 50,000 Assay Points
HTRF Human Total VAV1 Detection Kit, 50,000 Assay Points
Molecular Devices Absorbance Filter, 600 nm, 8 nm Bandwidth
The Molecular Devices absorbance bandpass filter centered at 600 nm with an 8 nm full width at half maximum (FWHM) bandwidth is a replacement optical filter for compatible microplate readers. It enables narrow-band absorbance measurements for endpoint and kinetic assays and installs as an interchangeable filter slide.
- Enables narrow-band absorbance measurements at 600 nm.
- 8 nm full width at half maximum bandpass for spectral selectivity.
- Designed for installation as an interchangeable filter slide in compatible microplate readers.
- Suitable for quantitative absorbance assays and endpoint or kinetic measurements.
- Replacement spare for instruments that accept interchangeable absorbance filters.
Molecular Devices Fluorescence Polarization Filter, 485 nm, 20 nm, Vertical
The Molecular Devices fluorescence polarization filter (MPN 6590-0094) is a vertical polarization filter engineered for fluorescence polarization assays with a 485 nm center wavelength and a 20 nm optical bandwidth. It is intended as a replacement or accessory filter for compatible multi-mode microplate readers.
- Optimized for fluorescence polarization assays at a 485 nm center wavelength.
- 20 nm bandwidth provides narrow spectral selection and reduced crosstalk.
- Vertical polarization orientation for polarization-based measurements.
- Designed for installation in filter-slide assemblies of compatible microplate readers.
- Replacement filter for restoring or modifying detection channels.
Revvity Health Sciences Inc HTRF Human & Mouse Total CHK-1 Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Total CHK-1 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total Cyclin D1 Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Total Cyclin D1 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Phospho-Total protein lysis buffer 6, 130 mL
HTRF Phospho-Total protein lysis buffer 6, 130 mL
Precision Glassblowing of Colorado TJA INJECTOR 1.0MM 12540701
Quartz Injector, 1.0mm (2 red rings), for TJA 61E, Iris & Intrepid Radial, and for TJA 61E Trace, Iris AP, & Intrepid