Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Promocell GmbH PromoCell 3D Tumorsphere Medium XF prf 250 ml, with C-39870 attached to flask
The PromoCell 3D Tumorsphere Medium XF is a culture system developed for the standardized serial culture of cancer cell lines as tumorspheres/mammospheres. The formulation supports sustained cell proliferation allowing for serial passage of the 3D culture. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials.Components:The PromoCell 3D Tumorsphere Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium.Key features:Establish 3D tumorsphere cultures directly from 2D cancer cell lines Suitable for long-term routine culture of tumorspheres Compatible with most commonly used cancer cell lines Xeno-free and serum-free formulation
Molecular Devices Microplate Reader, Multimode, 200-1000 nm, 6-384 Wells
The SpectraMax M2 is a dual-monochromator multimode microplate reader for absorbance, fluorescence, time-resolved fluorescence, and luminescence assays across 6-384 well plates and cuvette formats.
- Multimode detection for absorbance, fluorescence intensity, time-resolved fluorescence, and luminescence.
- Wavelength range: absorbance 200-1000 nm, fluorescence excitation 250-850 nm, fluorescence emission 360-850 nm.
- Supports 6, 12, 24, 48, 96, and 384 well plate formats.
- Dual-mode cuvette port for cuvette-based assays and dual monochromators for tunable wavelength selection.
- Power: 100-240 Vac, 3 A, 50/60 Hz; power consumption under 125 W.
- Includes PathCheck optical pathlength normalization, kinetic and endpoint read modes, and robot compatibility.
Molecular Devices Multi-Mode Microplate Reader, Dual Monochromator, 6-384-Well
The SpectraMax M3 multi-mode microplate reader is a configurable, high-performance instrument for absorbance, fluorescence, and luminescence assays, designed for both research and regulated laboratory environments. It combines tunable optics with flexible plate handling and compliance-capable analysis software.
- Dual monochromator optics, tunable in 1-nm increments
- Detection modes include absorbance, fluorescence, luminescence, fluorescence polarization, and time-resolved assays
- Supports 6- to 384-well microplates, with top and bottom reading options
- High-sensitivity detectors with xenon flash lamp illumination
- Software features secure data acquisition and 21 CFR Part 11-compatible controls
Molecular Devices Microplate Reader, 6-1536-Well, Dual PMT, User-Upgradeable
The SpectraMax Paradigm multi-mode microplate reader from Molecular Devices is a user-upgradeable detection platform for absorbance, fluorescence, time-resolved fluorescence, fluorescence polarization, and luminescence assays. It supports 6- to 1536-well plates and modular detection cartridges for configurable optics.
- User-upgradeable detection cartridges for configurable optics and modes.
- Supports 6- to 1536-well plate formats.
- Dual photomultiplier tubes for enhanced sensitivity and throughput.
- Wide absorbance range 230-1000 nm and fluorescence excitation/emission coverage.
- Integrated temperature control, plate shaking, and robotic compatibility.
- High dynamic range greater than six decades for quantitative assays.
- Compact footprint: 39 cm W x 45 cm H x 63 cm D.
Molecular Devices Microplate Reader, Multi-Mode, Monochromator, Temperature Control
The SpectraMax M5e multi-mode microplate reader provides flexible, high-performance detection for absorbance, fluorescence, luminescence, time-resolved fluorescence, fluorescence polarization, and HTRF assays across 6-384 well plates and cuvettes. It features a tunable monochromator with 1 nm spectral resolution, temperature control, and adaptive detector optimization for broad dynamic range.
- Supports absorbance, fluorescence, luminescence, time-resolved fluorescence, fluorescence polarization, and HTRF assays.
- Monochromator with 1 nm wavelength selection for spectral scanning and up to six wavelengths per read.
- Temperature control from ambient +2°C to 60°C for incubated assays.
- Automated detector optimization and absorbance normalization for extended dynamic range and improved accuracy.
- Compatible with GxP-capable data acquisition and validation workflows for regulated environments.